Wrenthorpe's bid for a record fifth successive Solly Sports Heavy Woollen Cup win was brought to a sudden end as they suffered an eight-wicket defeat at Delph & Dobcross.
And Barnsley, winners in 2005 and 2006, also made a first round exit after going down by 55 runs to Bradford League Division Two side Spen Victoria.
Wrenthorpe's four-year dominanation of the competition was finally ended on a wet pitch at Delph & Dobcross. Their normally free-scoring batsmen were made to struggle and a total of 118 was never going to seriously test their Huddersfield League opponents.
Greig Batty (30) was Wrenthorpe's top scorer while Faisal Butt picked up 5-24 for the home side. Butt followed up with an unbeaten knock of 40 as he and Irfan Zahoor (65no) steered their side home.
Barnsley's defeat at Spen Victoria, the venue for this year's final, was the biggest surprise of the day.
The Yorkshire League side had three Yorkshire players Oliver Hannon Dalby, Azeem Rafiq and Gary Ballance in their side and looked in control as they had the home side in deep trouble at 90-7 with Hannon Dalby taking 3-15.
But Spen's tail wagged vigorously with new recruit Joel Pollard blasting 53 and Craig Woodhead an unbeaten 30 as the home side boosted their total to a respectable 185 all out.
Barnsley lost wickets steadily in reply as Robert Warriner (3-22), Rob Burton (3-39) and Craig Woodhead (3-10) seized the initiative and dismissed the visitors for 130.
Townville's prolific opener Tim Walton produced the top individual innings of the day. He made 158 as his Central Yorkshire League side hit 396-4 in their 190-run win over Wakefield St Michael's.
One of the best games of the day was at Albert Terrace and saw Bradford League side Woodlands defeat Central Yorkshire League Methley by one wicket thanks to a four off the last ball by 18-year-old Scott Richardson.
Woodlands looked to have little chance of chasing down Methley's score of 251-5 when they lost their first three wickets with just seven runs on the board, but former skipper Tim Orrell (79) and Chris Brice (63) revived their side with a fifth-wicket stand of 127.
Big-hitting Sarfraz Ahmed blasted a rapid 44 and then Richardson steered his side home despite the valiant efforts of Sri Lankan Hemantha Alles (5-49).
Methley had built their total of 251-5 on the contributions of Hamid Khan (73), Chris Holliday (45no) and Alles (42)
The draw for the second round will be made at 7.45pm on Monday.